Review



easysep tm human whole blood cd66b positive selection kit  (STEMCELL Technologies Inc)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    STEMCELL Technologies Inc easysep tm human whole blood cd66b positive selection kit
    <t>CD66b+</t> neutrophils were predominantly located within the peritumoral stroma region in LSCC patients, which were associated with poorer prognosis. ( A ) Representative images were shown for CD45 and CD66b IHC staining of tumor tissues and normal tissues from LSCC patients. ( B ) Representative LSCC tumor tissues exhibited various density of CD66b+ neutrophils in peritumoral stroma and intratumoral regions. Bars = 50μm. ( C ) Infiltrating CD66b+ neutrophils in intratumoral and peritumoral region of tumor tissue, and epithelium and sub-epithelium region of normal tissues were analyzed. ( D and E ) LSCC patients with advanced T stage had more intratumoral and peritumoral infiltrated CD66b+ neutrophils. ( F – I ) Kaplan–Meier curves for overall survival and disease-free survival of 45 LSCC patients according to different density of CD66b+ neutrophils infiltrated in peritumoral stroma and intratumoral regions of tumor tissues. 50X, 200X, 400X: magnification of 50, 200, and 400 fold; *p < 0.05, **p < 0.01, ***p < 0.001.
    Easysep Tm Human Whole Blood Cd66b Positive Selection Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easysep+human+whole+blood+cd66b+positive+selection+kit/pe+conjugated+cd66b+antibody/pmc08859980-60-6-16
    Average 90 stars, based on 1 article reviews
    easysep tm human whole blood cd66b positive selection kit - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Tumor-Infiltrating PD-L1+ Neutrophils Induced by GM-CSF Suppress T Cell Function in Laryngeal Squamous Cell Carcinoma and Predict Unfavorable Prognosis"

    Article Title: Tumor-Infiltrating PD-L1+ Neutrophils Induced by GM-CSF Suppress T Cell Function in Laryngeal Squamous Cell Carcinoma and Predict Unfavorable Prognosis

    Journal: Journal of Inflammation Research

    doi: 10.2147/JIR.S347777

    CD66b+ neutrophils were predominantly located within the peritumoral stroma region in LSCC patients, which were associated with poorer prognosis. ( A ) Representative images were shown for CD45 and CD66b IHC staining of tumor tissues and normal tissues from LSCC patients. ( B ) Representative LSCC tumor tissues exhibited various density of CD66b+ neutrophils in peritumoral stroma and intratumoral regions. Bars = 50μm. ( C ) Infiltrating CD66b+ neutrophils in intratumoral and peritumoral region of tumor tissue, and epithelium and sub-epithelium region of normal tissues were analyzed. ( D and E ) LSCC patients with advanced T stage had more intratumoral and peritumoral infiltrated CD66b+ neutrophils. ( F – I ) Kaplan–Meier curves for overall survival and disease-free survival of 45 LSCC patients according to different density of CD66b+ neutrophils infiltrated in peritumoral stroma and intratumoral regions of tumor tissues. 50X, 200X, 400X: magnification of 50, 200, and 400 fold; *p < 0.05, **p < 0.01, ***p < 0.001.
    Figure Legend Snippet: CD66b+ neutrophils were predominantly located within the peritumoral stroma region in LSCC patients, which were associated with poorer prognosis. ( A ) Representative images were shown for CD45 and CD66b IHC staining of tumor tissues and normal tissues from LSCC patients. ( B ) Representative LSCC tumor tissues exhibited various density of CD66b+ neutrophils in peritumoral stroma and intratumoral regions. Bars = 50μm. ( C ) Infiltrating CD66b+ neutrophils in intratumoral and peritumoral region of tumor tissue, and epithelium and sub-epithelium region of normal tissues were analyzed. ( D and E ) LSCC patients with advanced T stage had more intratumoral and peritumoral infiltrated CD66b+ neutrophils. ( F – I ) Kaplan–Meier curves for overall survival and disease-free survival of 45 LSCC patients according to different density of CD66b+ neutrophils infiltrated in peritumoral stroma and intratumoral regions of tumor tissues. 50X, 200X, 400X: magnification of 50, 200, and 400 fold; *p < 0.05, **p < 0.01, ***p < 0.001.

    Techniques Used: Immunohistochemistry

    Immune cell infiltration profiles in LSCC. ( A ) Representative polychromatic dot plots displayed CD45+ leukocytes, CD3+ T cells, CD4+ T cells, CD8+ T cells γδT cells (γδTCR+) and neutrophils (CD66b+) in peripheral blood, tumor tissues and normal tissues from LSCC patients or healthy controls. ( B ) Calculative distribution of lymphocytes, neutrophils, NLR, CD3+T cells, CD4+T cells, CD8+T cells, γδT cells, and CD4+T cells /CD8 + T cells ratio in peripheral blood, tumor tissues and normal tissues. ( C ) Representative PD-1 expression on CD4+T/CD8+T/γδT cells and PDL-1 expression on CD66b+ neutrophils from the peripheral blood, tumor tissues and normal tissues. ( D ) Statistics analysis of PD-1+ CD4+T, PD-1+ CD8+T, PD-1+ γδT cells and PDL-1+ CD66b+ neutrophils. ( E ) Association analysis of TANs, NLR, CD3+ T cells or CD8+ T cells and tumor staging. ( F ) Linear correlation between TANs and CD3+ T cells, CD4+T cells, CD8+ T cells, or γδT cells. ( G ) Linear correlation between PD-L1+ TANs and PD-1+ CD4+ T cells, PD-1+ CD8+ T cells, or PD-1+ γδT cells. Each dot represents an independent data point as determined by flow cytometry. ns = p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001.
    Figure Legend Snippet: Immune cell infiltration profiles in LSCC. ( A ) Representative polychromatic dot plots displayed CD45+ leukocytes, CD3+ T cells, CD4+ T cells, CD8+ T cells γδT cells (γδTCR+) and neutrophils (CD66b+) in peripheral blood, tumor tissues and normal tissues from LSCC patients or healthy controls. ( B ) Calculative distribution of lymphocytes, neutrophils, NLR, CD3+T cells, CD4+T cells, CD8+T cells, γδT cells, and CD4+T cells /CD8 + T cells ratio in peripheral blood, tumor tissues and normal tissues. ( C ) Representative PD-1 expression on CD4+T/CD8+T/γδT cells and PDL-1 expression on CD66b+ neutrophils from the peripheral blood, tumor tissues and normal tissues. ( D ) Statistics analysis of PD-1+ CD4+T, PD-1+ CD8+T, PD-1+ γδT cells and PDL-1+ CD66b+ neutrophils. ( E ) Association analysis of TANs, NLR, CD3+ T cells or CD8+ T cells and tumor staging. ( F ) Linear correlation between TANs and CD3+ T cells, CD4+T cells, CD8+ T cells, or γδT cells. ( G ) Linear correlation between PD-L1+ TANs and PD-1+ CD4+ T cells, PD-1+ CD8+ T cells, or PD-1+ γδT cells. Each dot represents an independent data point as determined by flow cytometry. ns = p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001.

    Techniques Used: Expressing, Flow Cytometry

    Related Articles

    Purification:

    Article Title: Human Inflammatory Neutrophils Express Genes Encoding Peptidase Inhibitors: Production of Elafin Mediated by NF-κB and CCAAT/Enhancer-Binding Protein β.
    Article Snippet: .. Neutrophils were then purified by positive selection using the EasySep Human Whole Blood CD66b Positive Selection Kit (STEMCELL Technologies, Vancouver, BC, Canada). .. After incubations of neutrophils under appropriate conditions for 48 h and elimination of apoptotic cells (see above), LL neutrophils and their control counterparts (10–15 106 cells/ml) were homogenized in QIAzol lysis buffer (QIAGEN).

    Selection:

    Article Title: Human Inflammatory Neutrophils Express Genes Encoding Peptidase Inhibitors: Production of Elafin Mediated by NF-κB and CCAAT/Enhancer-Binding Protein β.
    Article Snippet: .. Neutrophils were then purified by positive selection using the EasySep Human Whole Blood CD66b Positive Selection Kit (STEMCELL Technologies, Vancouver, BC, Canada). .. After incubations of neutrophils under appropriate conditions for 48 h and elimination of apoptotic cells (see above), LL neutrophils and their control counterparts (10–15 106 cells/ml) were homogenized in QIAzol lysis buffer (QIAGEN).

    Article Title: Lack of Significant Elevation of Myeloid-Derived Suppressor Cells in Peripheral Blood of Chronically Hepatitis C Virus-Infected Individuals
    Article Snippet: .. PBMC were stained with the EasySep human whole blood CD66b positive selection kit (Stemcell Technologies, France) according to the manufacturer's protocol. ..

    Staining:

    Article Title: Lack of Significant Elevation of Myeloid-Derived Suppressor Cells in Peripheral Blood of Chronically Hepatitis C Virus-Infected Individuals
    Article Snippet: .. PBMC were stained with the EasySep human whole blood CD66b positive selection kit (Stemcell Technologies, France) according to the manufacturer's protocol. ..



    Similar Products

    easysep tm human whole blood cd66b positive selection kit  (STEMCELL Technologies Inc)
    90
    STEMCELL Technologies Inc easysep tm human whole blood cd66b positive selection kit
    <t>CD66b+</t> neutrophils were predominantly located within the peritumoral stroma region in LSCC patients, which were associated with poorer prognosis. ( A ) Representative images were shown for CD45 and CD66b IHC staining of tumor tissues and normal tissues from LSCC patients. ( B ) Representative LSCC tumor tissues exhibited various density of CD66b+ neutrophils in peritumoral stroma and intratumoral regions. Bars = 50μm. ( C ) Infiltrating CD66b+ neutrophils in intratumoral and peritumoral region of tumor tissue, and epithelium and sub-epithelium region of normal tissues were analyzed. ( D and E ) LSCC patients with advanced T stage had more intratumoral and peritumoral infiltrated CD66b+ neutrophils. ( F – I ) Kaplan–Meier curves for overall survival and disease-free survival of 45 LSCC patients according to different density of CD66b+ neutrophils infiltrated in peritumoral stroma and intratumoral regions of tumor tissues. 50X, 200X, 400X: magnification of 50, 200, and 400 fold; *p < 0.05, **p < 0.01, ***p < 0.001.
    Easysep Tm Human Whole Blood Cd66b Positive Selection Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easysep+human+whole+blood+cd66b+positive+selection+kit/pe+conjugated+cd66b+antibody/pmc08859980-60-6-16
    Average 90 stars, based on 1 article reviews
    easysep tm human whole blood cd66b positive selection kit - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    easysep human whole blood cd66b positive selection kit  (STEMCELL Technologies Inc)
    90
    STEMCELL Technologies Inc easysep human whole blood cd66b positive selection kit
    <t>CD66b+</t> neutrophils were predominantly located within the peritumoral stroma region in LSCC patients, which were associated with poorer prognosis. ( A ) Representative images were shown for CD45 and CD66b IHC staining of tumor tissues and normal tissues from LSCC patients. ( B ) Representative LSCC tumor tissues exhibited various density of CD66b+ neutrophils in peritumoral stroma and intratumoral regions. Bars = 50μm. ( C ) Infiltrating CD66b+ neutrophils in intratumoral and peritumoral region of tumor tissue, and epithelium and sub-epithelium region of normal tissues were analyzed. ( D and E ) LSCC patients with advanced T stage had more intratumoral and peritumoral infiltrated CD66b+ neutrophils. ( F – I ) Kaplan–Meier curves for overall survival and disease-free survival of 45 LSCC patients according to different density of CD66b+ neutrophils infiltrated in peritumoral stroma and intratumoral regions of tumor tissues. 50X, 200X, 400X: magnification of 50, 200, and 400 fold; *p < 0.05, **p < 0.01, ***p < 0.001.
    Easysep Human Whole Blood Cd66b Positive Selection Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easysep+human+whole+blood+cd66b+positive+selection+kit/pe+conjugated+cd66b+antibody/pm33762327-91-9-17
    Average 90 stars, based on 1 article reviews
    easysep human whole blood cd66b positive selection kit - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    magnetic microbeads easysep human whole blood cd66b positive selection kit  (STEMCELL Technologies Inc)
    90
    STEMCELL Technologies Inc magnetic microbeads easysep human whole blood cd66b positive selection kit
    <t>CD66b+</t> neutrophils were predominantly located within the peritumoral stroma region in LSCC patients, which were associated with poorer prognosis. ( A ) Representative images were shown for CD45 and CD66b IHC staining of tumor tissues and normal tissues from LSCC patients. ( B ) Representative LSCC tumor tissues exhibited various density of CD66b+ neutrophils in peritumoral stroma and intratumoral regions. Bars = 50μm. ( C ) Infiltrating CD66b+ neutrophils in intratumoral and peritumoral region of tumor tissue, and epithelium and sub-epithelium region of normal tissues were analyzed. ( D and E ) LSCC patients with advanced T stage had more intratumoral and peritumoral infiltrated CD66b+ neutrophils. ( F – I ) Kaplan–Meier curves for overall survival and disease-free survival of 45 LSCC patients according to different density of CD66b+ neutrophils infiltrated in peritumoral stroma and intratumoral regions of tumor tissues. 50X, 200X, 400X: magnification of 50, 200, and 400 fold; *p < 0.05, **p < 0.01, ***p < 0.001.
    Magnetic Microbeads Easysep Human Whole Blood Cd66b Positive Selection Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/easysep+human+whole+blood+cd66b+positive+selection+kit/pe+conjugated+cd66b+antibody/10__1186_slash_s41231___019___0052___5-63-17-25
    Average 90 stars, based on 1 article reviews
    magnetic microbeads easysep human whole blood cd66b positive selection kit - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    CD66b+ neutrophils were predominantly located within the peritumoral stroma region in LSCC patients, which were associated with poorer prognosis. ( A ) Representative images were shown for CD45 and CD66b IHC staining of tumor tissues and normal tissues from LSCC patients. ( B ) Representative LSCC tumor tissues exhibited various density of CD66b+ neutrophils in peritumoral stroma and intratumoral regions. Bars = 50μm. ( C ) Infiltrating CD66b+ neutrophils in intratumoral and peritumoral region of tumor tissue, and epithelium and sub-epithelium region of normal tissues were analyzed. ( D and E ) LSCC patients with advanced T stage had more intratumoral and peritumoral infiltrated CD66b+ neutrophils. ( F – I ) Kaplan–Meier curves for overall survival and disease-free survival of 45 LSCC patients according to different density of CD66b+ neutrophils infiltrated in peritumoral stroma and intratumoral regions of tumor tissues. 50X, 200X, 400X: magnification of 50, 200, and 400 fold; *p < 0.05, **p < 0.01, ***p < 0.001.

    Journal: Journal of Inflammation Research

    Article Title: Tumor-Infiltrating PD-L1+ Neutrophils Induced by GM-CSF Suppress T Cell Function in Laryngeal Squamous Cell Carcinoma and Predict Unfavorable Prognosis

    doi: 10.2147/JIR.S347777

    Figure Lengend Snippet: CD66b+ neutrophils were predominantly located within the peritumoral stroma region in LSCC patients, which were associated with poorer prognosis. ( A ) Representative images were shown for CD45 and CD66b IHC staining of tumor tissues and normal tissues from LSCC patients. ( B ) Representative LSCC tumor tissues exhibited various density of CD66b+ neutrophils in peritumoral stroma and intratumoral regions. Bars = 50μm. ( C ) Infiltrating CD66b+ neutrophils in intratumoral and peritumoral region of tumor tissue, and epithelium and sub-epithelium region of normal tissues were analyzed. ( D and E ) LSCC patients with advanced T stage had more intratumoral and peritumoral infiltrated CD66b+ neutrophils. ( F – I ) Kaplan–Meier curves for overall survival and disease-free survival of 45 LSCC patients according to different density of CD66b+ neutrophils infiltrated in peritumoral stroma and intratumoral regions of tumor tissues. 50X, 200X, 400X: magnification of 50, 200, and 400 fold; *p < 0.05, **p < 0.01, ***p < 0.001.

    Article Snippet: PANs and TANs were isolated with EasySep TM human whole blood CD66b positive selection kit (18,682, StemCell Technologies, Vancouver, Canada) according to the manufacturer’s instructions.

    Techniques: Immunohistochemistry

    Immune cell infiltration profiles in LSCC. ( A ) Representative polychromatic dot plots displayed CD45+ leukocytes, CD3+ T cells, CD4+ T cells, CD8+ T cells γδT cells (γδTCR+) and neutrophils (CD66b+) in peripheral blood, tumor tissues and normal tissues from LSCC patients or healthy controls. ( B ) Calculative distribution of lymphocytes, neutrophils, NLR, CD3+T cells, CD4+T cells, CD8+T cells, γδT cells, and CD4+T cells /CD8 + T cells ratio in peripheral blood, tumor tissues and normal tissues. ( C ) Representative PD-1 expression on CD4+T/CD8+T/γδT cells and PDL-1 expression on CD66b+ neutrophils from the peripheral blood, tumor tissues and normal tissues. ( D ) Statistics analysis of PD-1+ CD4+T, PD-1+ CD8+T, PD-1+ γδT cells and PDL-1+ CD66b+ neutrophils. ( E ) Association analysis of TANs, NLR, CD3+ T cells or CD8+ T cells and tumor staging. ( F ) Linear correlation between TANs and CD3+ T cells, CD4+T cells, CD8+ T cells, or γδT cells. ( G ) Linear correlation between PD-L1+ TANs and PD-1+ CD4+ T cells, PD-1+ CD8+ T cells, or PD-1+ γδT cells. Each dot represents an independent data point as determined by flow cytometry. ns = p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001.

    Journal: Journal of Inflammation Research

    Article Title: Tumor-Infiltrating PD-L1+ Neutrophils Induced by GM-CSF Suppress T Cell Function in Laryngeal Squamous Cell Carcinoma and Predict Unfavorable Prognosis

    doi: 10.2147/JIR.S347777

    Figure Lengend Snippet: Immune cell infiltration profiles in LSCC. ( A ) Representative polychromatic dot plots displayed CD45+ leukocytes, CD3+ T cells, CD4+ T cells, CD8+ T cells γδT cells (γδTCR+) and neutrophils (CD66b+) in peripheral blood, tumor tissues and normal tissues from LSCC patients or healthy controls. ( B ) Calculative distribution of lymphocytes, neutrophils, NLR, CD3+T cells, CD4+T cells, CD8+T cells, γδT cells, and CD4+T cells /CD8 + T cells ratio in peripheral blood, tumor tissues and normal tissues. ( C ) Representative PD-1 expression on CD4+T/CD8+T/γδT cells and PDL-1 expression on CD66b+ neutrophils from the peripheral blood, tumor tissues and normal tissues. ( D ) Statistics analysis of PD-1+ CD4+T, PD-1+ CD8+T, PD-1+ γδT cells and PDL-1+ CD66b+ neutrophils. ( E ) Association analysis of TANs, NLR, CD3+ T cells or CD8+ T cells and tumor staging. ( F ) Linear correlation between TANs and CD3+ T cells, CD4+T cells, CD8+ T cells, or γδT cells. ( G ) Linear correlation between PD-L1+ TANs and PD-1+ CD4+ T cells, PD-1+ CD8+ T cells, or PD-1+ γδT cells. Each dot represents an independent data point as determined by flow cytometry. ns = p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001.

    Article Snippet: PANs and TANs were isolated with EasySep TM human whole blood CD66b positive selection kit (18,682, StemCell Technologies, Vancouver, Canada) according to the manufacturer’s instructions.

    Techniques: Expressing, Flow Cytometry